DNA Fragment size selection

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Size selection using the Blue Pippin






The Blue Pippin features the ability to collect narrow and even fragment distributions as well as the flexibility to collect wide ranges of fragments. It also has the benefit of using pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. The versatility of size ranges that can be collected by the instrument make it ideal for NGS library preparation pipelines related to short libraries like the Illumina and Ion Torrent platforms as well as large libraries like those used in the PacBio and Oxford Nanopore platforms. Target sizes or ranges of sizes are entered in the software, fractions of interest are collected in an elution buffer, which is then provided to the customer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user. At $25 a lane, the different cassettes and size ranges available are:

3% agarose, 100-250bp

2% agarose, 100-600bp

1.5% agarose, 250bp – 1.5kb

0.75% agarose, 1-50kb

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