The Genome Analysis Core conducts several RT-PCR platforms plus single cell encapsulation for RNA Seq 3’ library production.
The BioMark Genetic Analysis Platform is a system that is a fully integrated system enabling analysis of gene expression, genotyping, mutant detection, and absolute quantization of nucleic-acid sequences utilizing Dynamic Array Integrated Fluidic Chip (IFC) technology which provides high-throughput real-time qPCR.
The BioRad QX200 Droplet Digital PCR (ddPCR) provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
- PCR-positive and PCR-negative droplets
- Provide absolute quantification of target DNA in digital form.
The Applied Biosystems StepOnePlus RT-PCR is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications
The BioRad ddSEQ™ Single-Cell Isolator in collaboration with Illumina can provide 3’ mRNA single cell RNA Seq libraries by utilizing disposable microfluidic cartridges to coencapsulate single cells and barcodes into subnanoliter droplets, where cell lysis, barcoding, and 3’ RNA Seq libraries can occur.
Genome Analysis also provides other services such as RNA quality control, cDNA services, and electroporation using the Lonza Nucleofector system.
Location: EBB Building, Room 2227A