Mass spectrometry (MS) is an analytical technique for the determination of the elemental composition of a sample or molecule. It is also used for elucidating the chemical structures of molecules, such as peptides and other chemical compounds. The MS principle consists of ionizing chemical compounds to generate charged molecules or molecule fragments and measurement of their mass-to-charge ratios.
This facility currently houses five mass spectrometers. Two of these instruments are recently acquired state-of-the-art tandem mass spectrometers. These instruments are an ABI QSTAR-XL of hybrid Q-TOF configuration and an ABI 4700 MALDI TOF/TOF. These instruments provide a unique and complimentary mass spectrometry capability with regard to both ionization and dissociation of widely different molecular species. The QSTAR is equipped with capillary LC pumps, autosampler, as well as microswitching and fractioning devices enabling analysis of complex mixtures with very high sensitivity. The QSTAR has both an electrospray and dynamic nanospray source for generation of intact molecular ions directly from solution. Both MS and MS/MS data are acquired with high resolution (> 7000 FWHM) and accurate mass measurement (< 10 ppm) for unequivocal molecular identification and structure elucidation. MS/MS on this instrument is performed via low energy collisions (CID) analogous to that of a triple quadrupole. The TOF/TOF generates ions via matrix assisted laser desorption (MALDI) with a 200Hz laser. This enables either a 10-fold increase in sample throughput or data collection vs other MALDI instruments. This instrument is equipped with both linear and reflecting detectors. The former is used for large molecular mass species (> 10 kDa) with low resolution, and the latter is used for high resolution, accurate mass measurement of lower molecular mass species (< 10 kDa). The ABI 4700 is a true tandem mass spectrometer whereby MS/MS is performed via precursor ion selection with subsequent dissociation by either metastable or high energy collision induced dissociation.
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Location: Petit Institute