Our research focuses on LC-MS based proteomics and its biomedical applications. Novel MS based methods are being developed to characterize proteins, especially protein post-translational modifications (PTMs). MS-based large-scale analysis can systematically identify and quantify proteins and their PTMs for the different states of cells or tissues, such as cancerous samples. This may provide a better understanding of the function of proteins, the role that proteins play in physiological and pathological processes, cell signaling, cell metabolism, and the relationship between proteins and cancer.
My research group specializes in analytical mass spectrometry, with an emphasis on fundamental studies, instrument development and applications in biomedical and chemical evolution research. We are currently working along four main fields of work: a) metabolomics of ovarian cancer, prostate cancer and cystic fibrosis with an emphasis on diagnostics, b) development of new instrumentation for molecular imaging using mass spectrometry, c) development of new ambient ion generation approaches and d) ion mobility gas-phase separations enhancing imaging and metabolomic studies.
Development of metal specific fluorescent probes, mechanistic study of metalloprotein catalyzed reactions with unusual coordination geometries, development of protein-based, semisynthetic organometallic catalysts in aqueous solution
Research in the Barry group is focused on how the dynamic and responsive protein matrix facilitates biological catalysis. We use a wide range of high resolution spectroscopic, biochemical, and structural techniques to describe the reaction coordinate, which reveals the motion of the protein in space and time. ��
Professor Peralta-Yahya's research group is developing foundational technologies to more rapidly and effectively engineer biological systems for chemical synthesis. One area of research is the development of biosensors to screen chemical-producing microbes, which could identify strains that produce chemicals at industrially relevant yield. This technology has potential applications in the area of microbial synthesis of pharmaceuticals & microbial production of high energy density fuels.
Professor May is interested in rational, molecularly based approaches to problems in neurochemistry, in the development of novel enzyme effectors such as suicide substrates and transition state analogs, and in basic mechanistic studies on enzymes involved in the biosynthesis, metabolism, interconversion and regulation of neurotransmitters, neuroregulators and biologically active neuropeptides and peptide hormones. Both chemical and physical techniques are being used to investigate the structure and reactivity of these enzymes, and detailed stereochemical and structural studies on substrates, products, and inhibitors are being pursued. In addition, pharmacological techniques are being utilized in order to evaluate the action of novel enzyme effectors on cardiovascular and neurological functions. Dr. May's group has established a special cell culture laboratory which is used extensively in their neurochemical work.